Online liquid chromatography-tandem mass spectrometry cyanide determination in blood.
Identifieur interne : 000678 ( France/Analysis ); précédent : 000677; suivant : 000679Online liquid chromatography-tandem mass spectrometry cyanide determination in blood.
Auteurs : C. Lacroix [France] ; E. Saussereau ; F. Boulanger ; J P GoulléSource :
- Journal of analytical toxicology [ 1945-2403 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Cyanides, Hazardous Substances.
- chemical , chemistry : Cyanides.
- instrumentation : Blood Chemical Analysis.
- methods : Blood Chemical Analysis.
- Chromatography, Liquid, Forensic Toxicology, Humans, Online Systems, Tandem Mass Spectrometry.
Abstract
An original liquid chromatography-tandem mass spectrometry (LC-MS-MS) method coupled to online extraction has been developed for cyanide determination in blood. A method involving fluorimetric detection after naphthalene-2,3-dicarboxyaldehyde (NDA) complexation by taurine in the presence of cyanide was previously described. Its performance was limited because of the absence of an internal standard (IS). Using cyanide isotope (13)C(15)N as IS allowed quantification in MS-MS. After the addition of (13)C(15)N, 25 μL of blood were diluted in water and deproteinized with methanol. Following derivatization with NDA and taurine for 10 min at 4°C, 100 μL was injected into the online LC-MS-MS system. An Oasis HLB was used as an extraction column, and a C18 Atlantis was the analytical column. The chromatographic cycle was performed with an ammonium formate (20 mM, pH 2.8) (solvent A) and acetonitrile/solvent A (90:10, v/v) gradient in 6 min. Detection was performed in negative electrospray ionization mode (ESI(-)) with a Quattro Micro. For quantification, transitions of derivatives formed with CN and (13)C(15)N were monitored, respectively, as follows: 299.3/191.3 and 301.3/193.3. The procedure was fully validated, linear from 26 to 2600 ng/mL with limit of detection of 10 ng/mL. This method, using a small blood sample, is not only simple, but also time saving. The specificity and sensitivity of LC-MS-MS coupled to online extraction and using (13)C(15)N as the IS make this method very suitable for cyanide determination in blood and could be useful in forensic toxicology.
PubMed: 21439149
Affiliations:
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pubmed:21439149Le document en format XML
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Saussereau</name></author><author><name sortKey="Boulanger, F" sort="Boulanger, F" uniqKey="Boulanger F" first="F" last="Boulanger">F. Boulanger</name></author><author><name sortKey="Goulle, J P" sort="Goulle, J P" uniqKey="Goulle J" first="J P" last="Goullé">J P Goullé</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21439149</idno><idno type="pmid">21439149</idno><idno type="wicri:Area/PubMed/Corpus">000214</idno><idno type="wicri:Area/PubMed/Curation">000214</idno><idno type="wicri:Area/PubMed/Checkpoint">000214</idno><idno type="wicri:Area/Ncbi/Merge">000285</idno><idno type="wicri:Area/Ncbi/Curation">000285</idno><idno type="wicri:Area/Ncbi/Checkpoint">000285</idno><idno type="wicri:Area/Main/Merge">000786</idno><idno type="wicri:Area/Main/Curation">000785</idno><idno type="wicri:Area/Main/Exploration">000785</idno><idno type="wicri:Area/France/Extraction">000678</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Online liquid chromatography-tandem mass spectrometry cyanide determination in blood.</title><author><name sortKey="Lacroix, C" sort="Lacroix, C" uniqKey="Lacroix C" first="C" last="Lacroix">C. Lacroix</name><affiliation wicri:level="3"><nlm:affiliation>Pharmacokinetic and Toxicology Laboratory, J Monod Hospital, F-76083 Le Havre Cedex, France. christian.lacroix@ch-havre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Pharmacokinetic and Toxicology Laboratory, J Monod Hospital, F-76083 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Saussereau, E" sort="Saussereau, E" uniqKey="Saussereau E" first="E" last="Saussereau">E. Saussereau</name></author><author><name sortKey="Boulanger, F" sort="Boulanger, F" uniqKey="Boulanger F" first="F" last="Boulanger">F. Boulanger</name></author><author><name sortKey="Goulle, J P" sort="Goulle, J P" uniqKey="Goulle J" first="J P" last="Goullé">J P Goullé</name></author></analytic><series><title level="j">Journal of analytical toxicology</title><idno type="eISSN">1945-2403</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood Chemical Analysis (instrumentation)</term><term>Blood Chemical Analysis (methods)</term><term>Chromatography, Liquid</term><term>Cyanides (blood)</term><term>Cyanides (chemistry)</term><term>Forensic Toxicology</term><term>Hazardous Substances (blood)</term><term>Humans</term><term>Online Systems</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Cyanides</term><term>Hazardous Substances</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cyanides</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Blood Chemical Analysis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blood Chemical Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Liquid</term><term>Forensic Toxicology</term><term>Humans</term><term>Online Systems</term><term>Tandem Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An original liquid chromatography-tandem mass spectrometry (LC-MS-MS) method coupled to online extraction has been developed for cyanide determination in blood. A method involving fluorimetric detection after naphthalene-2,3-dicarboxyaldehyde (NDA) complexation by taurine in the presence of cyanide was previously described. Its performance was limited because of the absence of an internal standard (IS). Using cyanide isotope (13)C(15)N as IS allowed quantification in MS-MS. After the addition of (13)C(15)N, 25 μL of blood were diluted in water and deproteinized with methanol. Following derivatization with NDA and taurine for 10 min at 4°C, 100 μL was injected into the online LC-MS-MS system. An Oasis HLB was used as an extraction column, and a C18 Atlantis was the analytical column. The chromatographic cycle was performed with an ammonium formate (20 mM, pH 2.8) (solvent A) and acetonitrile/solvent A (90:10, v/v) gradient in 6 min. Detection was performed in negative electrospray ionization mode (ESI(-)) with a Quattro Micro. For quantification, transitions of derivatives formed with CN and (13)C(15)N were monitored, respectively, as follows: 299.3/191.3 and 301.3/193.3. The procedure was fully validated, linear from 26 to 2600 ng/mL with limit of detection of 10 ng/mL. This method, using a small blood sample, is not only simple, but also time saving. The specificity and sensitivity of LC-MS-MS coupled to online extraction and using (13)C(15)N as the IS make this method very suitable for cyanide determination in blood and could be useful in forensic toxicology.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Boulanger, F" sort="Boulanger, F" uniqKey="Boulanger F" first="F" last="Boulanger">F. Boulanger</name><name sortKey="Goulle, J P" sort="Goulle, J P" uniqKey="Goulle J" first="J P" last="Goullé">J P Goullé</name><name sortKey="Saussereau, E" sort="Saussereau, E" uniqKey="Saussereau E" first="E" last="Saussereau">E. Saussereau</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Lacroix, C" sort="Lacroix, C" uniqKey="Lacroix C" first="C" last="Lacroix">C. Lacroix</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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